Studied the optimum extraction process of polysaccharide from Phaeoporus obliquus and the effect of Phaeoporus obliquus polysaccharide on carbon tetrachloride (CCl4)- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysaccharide were extraction power and time, which was a kind of pyran glucose by infrared spectroscopy. CCl4 and alcohol were employed respectively to establish CCl4 and alcohol-induced acute liver injury mouse models. Compared with model groups mice, Phaeoporus obliquus polysaccharide treatment at the doses of 100mg/kg and 200mg/kg exhibited an obvious reduction liver index, ALP, ALT, AST levels, MDA content and TNF-α level (p<0.01) and SOD activity was increased, which was in a dose-dependent manner. Compared with the model group, the necrosis degree of hepatocytes was obviously reduced and the small fat droplets were formed in some cytoplasm, especially in high dose group, which the liver cells recovered to the level of normal group. Rt-PCR results showed that the expression of CYP2E1 mRNA in liver tissues of Phaeoporus obliquus polysaccharide groups were significantly reduced, and the difference were statistically significant compared with the model group (p<0.05). These results demonstrated that Phaeoporus obliquus polysaccharide has significantly hepatoprotective effect on CCl4 and alcohol-induced acute liver injury in mice.
Chemical and Drug Induced Liver Injury/metabolism , Fungal Polysaccharides/pharmacology , Hepatocytes/drug effects , Inonotus , Liver Diseases, Alcoholic/metabolism , Liver/drug effects , Alanine Transaminase/drug effects , Alanine Transaminase/metabolism , Alkaline Phosphatase/drug effects , Alkaline Phosphatase/metabolism , Animals , Aspartate Aminotransferases/drug effects , Aspartate Aminotransferases/metabolism , Carbon Tetrachloride/toxicity , Central Nervous System Depressants/toxicity , Cytochrome P-450 CYP2E1/drug effects , Cytochrome P-450 CYP2E1/genetics , Ethanol/toxicity , Hepatocytes/metabolism , Hepatocytes/pathology , Liver/metabolism , Liver/pathology , Malondialdehyde/metabolism , Mice , RNA, Messenger/drug effects , RNA, Messenger/metabolism , Superoxide Dismutase/drug effects , Superoxide Dismutase/metabolism , Tumor Necrosis Factor-alpha/drug effects , Tumor Necrosis Factor-alpha/metabolism
OBJECTIVE@#To explore the effect of nutrient support on severe infant pneumonia.@*METHODS@#Prospective study was conducted on the outcome of 567 inpatients suffering from severe pneumonia in 13 hospitals randomly selected in Hunan. Twelve factors were surveyed and data analyzed by multiple logistic regression.@*RESULTS@#Malnnutrition, anemia and rickets were risk factors in severe pneumonia, and nutrient support had protective effect on severe pneumonia.@*CONCLUSION@#Nutrient support contributes to the positive outcome of severe infant pneumonia.
Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Enteral Nutrition , Nutritional Support , Parenteral Nutrition , Pneumonia , Diet Therapy , Therapeutics , Prospective Studies , Treatment Outcome , gamma-Globulins , Therapeutic Uses
